In 1984, Kary Mullis invented the Polymerase Chain Reaction Technology. This technology subsequently gave birth to a PCR machine. With the Real-Time PCR, which involves real-time gene detection and expression analysis, the bioscience field has been revolutionized in the 21st century. Its tremendous applications have changed the world with services that include early disease diagnosis, forensic analysis, quantitative genotyping, genetic variation checks in various organisms, and so on.
Understanding the Necessity of Real-Time PCR
Every bit of bioscience advancement over the last century has been instrumental in the understanding of vital information. This information has been mostly about gene module network interaction which has been responsible for integrated cellular functions occurring coordinately in an isolated fashion. For an in-depth understanding of metabolism regulation, recognition of DNA is required which is possible with PCR.
Gene expression has been used actively to understand the relationship between cellular patterns and disease phenotypes. PCR-based detection technology has been very useful as research tools to define and identify enhanced information on various biological and microbial interactions. Many PCR methods have been developed to detect mRNA and quantify it in numerous organisms under consideration.
While Real-Time PCR Machine is highly specific and sensitive to conditions, it is the prime choice of scientists to detect the various gene expressions and understand the respective dynamics in different organizational associations in living beings.
Things that have helped realize the Importance of Real-Time PCR
- Obligate Pathogen Detection: Quantitative genotyping, detection of single nucleotide polymorphism, and genetic variations are possible to measure with Real-Time PCR.
- Multiplexing: Enables multiple gene identification with the help of fluorochromes and melting curves analysis. More sensitive in duplex reactions and hence better as an alternative over standard PCR.
- Gathering PCR Inhibitory Compounds: Involves factors influencing polymerase chain reaction.
- Steps involved that lead to the final PCR-
- Sampling: This step is important as it validates the analytical methods.
- Extraction of Nucleic acid
- Purification of Nucleic acid extracted
- PCR data output
When diving into the theory and understanding of the purpose of having a PCR machine, the importance of its Real-Time aspect is realized. The data achieved from RT PCR can provide the type of information previously achievable only with multiple conventional PCRs.
Areas where Real-Time PCR is utilized and Importance realized today
- Medical Science: With current technology, amplification in real-time can be detected to be utilized in personalized clinical diagnostics.
- Cancer: Accumulation of polymorphism and mutation is the cause of Cancer all of which can be diagnosed with RT PCR.
- Virology: Detection and quantification of viruses in human specimens are possible with major research through RT PCR.
- Bacteriology: Antibiotic theory was based on Gram stain classification. This was fastened by RT PCR which is a better alternative to conventional slow methods.
- Fungi: Detection and quantification of infectious fungi have been made rapidly with RT PCR.
- Protozoa: Facilitates the study of populations with parasitic infections. Detects amoebic dysentery, Chagas’ disease, giardiasis, immune-compromised patients, and so on.
- Veterinary: Viruses, Mycoplasma, and Bacterial detection and validation with RT PCR done at a fast pace.
Real-Time PCR Systems is a very common tool to detect and measure the gene expression profile. Over the past couple of years, the use of PCR product detection technology and application in real-time has seen a positive leap. Real-Time PCR is a tool that clarifies many problems in various aspects. This technology is the result of the stimulating needs in the clinical application of molecular methods. This is a truly important development in diagnostics in tremendous branches of science.